MYC transcription factors have well-defined roles in cellular transformation and generally serve as context-dependent transcriptional activators and repressors. Despite being one of the most intensively studied oncoproteins, the function of MYC remains controversial and even its core roles in transcriptional regulation and pause-release/elongation are in question. Ten years ago we identified MYC as a key modulator of pluripotent stem cell status and since then, MYC has been shown to be a critical determinant of cellular reprogramming. In pluripotent stem cells (PSCs) MYC is critical for self-renewal and it achieves this by directly regulating in excess of 1,000 genes. To gain new insights into how MYC regulates transcription we have taken a new approach by asking if it performs roles in the organization of chromosome architecture around target genes in conjunction with its recruitment to E-boxes. Data will be presented describing how MYC impacts the epigenetic landscape around target genes in stem cells and then, how this depends on chromosome architecture using 5c chromatin conformation capture analysis.