Poster Presentation 27th Lorne Cancer Conference 2015

Cross-presentation of cutaneous melanoma antigen by migratory XCR1+CD103- and XCR1+CD103+ dendritic cells (#299)

Ben Wylie 1 , Elke Seppanen 1 , Kun Xiao 2 , Rachael Zemek 1 , Damien Zanker 2 , Sandro Prato 3 , Bree Foley 1 , Prue H Hart 1 , Richard A Kroczek 4 , Weisan Chen 2 , Jason Waithman 1
  1. Telethon Kids Institute, Subiaco, WA, Australia
  2. La Trobe University, Melbourne, VIC, Australia
  3. CSL Limited, Bio21 Institute, Melbourne, VIC, Australia
  4. Robert Koch Institute, Berlin, Germany

The question of which dendritic cells (DCs) cross-present peripheral tumor antigens remains unanswered. We assessed the ability of multiple skin-derived and lymphoid resident DCs to perform this function in a novel orthotopic murine melanoma model where tumor establishment and expansion is within the skin. Two migratory populations defined as CD103-XCR1+ and CD103+XCR1+ efficiently cross-presented melanoma-derived antigen, with the CD103-XCR1+ DCs surprisingly dominating this process. These results are critical for understanding how anti-tumor CD8+ T cell immunity is coordinated to tumor antigens present within the skin.